Reporter Mice for Live Imaging

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LARGE has recently developed a number of fluorescent reporter mouse lines useful for live imaging of embryos and tissues to aid better understanding of dynamic developmental processes. The following list of reporter mice are available to the scientific community. Please contact "mutant.bdr(at)riken.jp" to request the reporter mouse line of your interest.

Download Excel File

contact

Mouse Request: mutant.bdr(at)riken.jp

Applications

Line names

CDB Acc. No.

Ref.

Cell organelles

Nucleus

R26R-H2B-EGFP

CDB0203K

1

R26-H2B-EGFP

CDB0238K

1

R26R-H2B-mCherry

CDB0204K

1

R26-H2B-mCherry

CDB0239K

1

R26R-H2B-mKeima-Red

CDB0205K

1

R26-H2B-mKeima-Red

CDB0240K

1

Membrane

R26R-Display-Kikume Green Red

CDB0207K

1

R26-Display-Kikume Green Red

CDB0242K

1

R26R-Display-Venus

CDB0208K

1

R26-Display-Venus

CDB0243K

1

R26R-Display-mCherry

CDB0209K

1

R26-Display-mCherry

CDB0244K

1

R26R-Lyn-Venus

CDB0219K

1

R26-Lyn-Venus

CDB0254K

1

R26R-Lyn-mCherry

CDB0278K

12

R26-Lyn-mCherry

CDB0287K

12

Nucleus & Membrane

R26R-RG

CDB0227K

2

R26-RG

CDB0237K

2

Golgi

R26R-Golgi-EGFP

CDB0211K

1

R26-Golgi-EGFP

CDB0246K

1

R26R-Golgi-mCherry

CDB0212K

1

R26-Golgi-mCherry

CDB0247K

1

Mitochondria

R26R-Mito-EGFP

CDB0216K

1

R26-Mito-EGFP

CDB0251K

1

Cytosol

CAG-KikGR-1

CDB0201T-1

4

CAG-KikGR-2

CDB0201T-2

4

 

Microtubule

R26R-EGFP-Tuba

CDB0210K

1

R26-EGFP-Tuba

CDB0245K

1

R26R-EB1-EGFP

CDB0213K

1

R26-EB1-EGFP

CDB0248K

1

R26R-hEMTB-EGFP

CDB0214K

1

R26-hEMTB-EGFP

CDB0249K

1

Actin

R26R-Venus-Moesin

CDB0217K

1

R26-Venus-Moesin

CDB0252K

1

R26R-Venus-Actin

CDB0218K

1

R26-Venus-Actin

CDB0253K

1

R26R-EGFP-Toriobp4

CDB0280K

 

R26-EGFP-Toriobp4

CDB0289K

 
 

Focal contacts

R26R-EGFP-Paxillin

CDB0221K

1

R26-EGFP-Paxillin

CDB0256K

1

 

Tight junction

R26R-ZO1-EGFP

CDB0225K

12

R26-ZO1-EGFP

CDB0260K

12

 

Adherence junction

R26R-PHA7-EGFP

CDB0226K

9

R26-PHA7-EGFP

CDB0261K

9

Cell cycle

Fucci2 (G1)

R26R-mCherry-hCdt1(30/120)

CDB0229K

3

R26-mCherry-hCdt1(30/120)

CDB0264K

3

Fucci2 (S/G2/M)

R26R-mVenus-hGem(1/110)

CDB0230K

3

R26-mVenus-hGem(1/110)

CDB0265K

3

Fucci2 (G1 & S/G2/M)

R26p-Fucci2

CDB0203T

3

 

Wnt signaling pathway

R26-WntVis

CDB0303K

8

ERK FRET biosensor

R26R-EKAREV-NES

CDB0306K

10

ERK FRET biosensor

R26R-EKAREV-NLS

CDB0307K

10

Cell lineage

R26R-ManGeKyou(R26R-MGK)

CDB0222K

11

Cell lineage

R26R-EGFP

CDB0297K

11

Cell lineage

R26R-nKikGR

CDB0312K

13 

Cell lineage

R26-nKikGR

CDB0318K

13 

Publications

1) Establishment of conditional reporter mouse lines at ROSA26 locus for live cell imaging.
Abe T, Kiyonari H, Shioi G, Inoue K, Nakao K, Aizawa S, Fujimori T.
Genesis. 2011 Jul;49(7):579-90.
PMID: 21445964


2) A mouse reporter line to conditionally mark nuclei and cell membranes for in vivo live-imaging.
Shioi G, Kiyonari H, Abe T, Nakao K, Fujimori T, Jang CW, Huang CC, Akiyama H, Behringer RR, Aizawa S.
Genesis. 2011 Jul;49(7):570-8.
PMID: 21504045


3) Visualization of cell cycle in mouse embryos with Fucci2 reporter directed by Rosa26 promoter.
Abe T, Sakaue-Sawano A, Kiyonari H, Shioi G, Inoue K, Horiuchi T, Nakao K, Miyawaki A, Aizawa S, Fujimori T.
Development. 2013 Jan 1;140(1):237-46.
PMID: 23175634

4) Blastocyst axis is specified independently of early cell lineage but aligns with the ZP shape.
Kurotaki Y1, Hatta K, Nakao K, Nabeshima Y, Fujimori T.
Science. 2007 May 4;316(5825):719-23.
PMID: 17446354


5) Reporter mouse lines for fluorescence imaging.
Abe T, Fujimori T.
Dev Growth Differ. 2013 May;55(4):390-405. Review.
PMID: 23621623


6) Live imaging of early mouse embryos using fluorescently labeled transgenic mice.
Abe T, Aizawa S, Fujimori T.
Methods Mol Biol. 2013;1052:101-8.
PMID: 23674079


7) Synergistic action of nectins and cadherins generates the mosaic cellular pattern of the olfactory epithelium.
Katsunuma S, Honda H, Shinoda T, Ishimoto Y, Miyata T, Kiyonari H, Abe T, Nibu K, Takai Y, Togashi H.
J Cell Biol. 2016 Feb 29;212(5):561-75.
PMID: 26929452


8) R26-WntVis reporter mice showing graded response to Wnt signal levels.
Takemoto T, Abe T, Kiyonari H, Nakao K, Furuta Y, Suzuki H, Takada S, Fujimori T, Kondoh H.
Genes Cells. 2016 Jun;21(6):661-9.
PMID: 27030109

9) Apical constriction in distal visceral endoderm cells initiates global, collective cell rearrangement in embryonic visceral endoderm to form anterior visceral endoderm.
Shioi, G., Hoshino, H., Abe, T., Kiyonari, H., Nakao, K., Meng, W., Furuta, Y., Fujimori, T., and Aizawa, S.
Developmental biology 2017 Sep 1;429(1):20-30
PMID: 28712875

10) Live-cell FRET imaging reveals a role of extracellular signal-regulated kinase activity dynamics in thymocyte motility
Konishi, Y., Terai, K., Fruta, Y., Kiyonari, H., Abe, T., Ueda, Y., Kinashi, T., Hamazaki, Y., Takaori-Kondo, A., and Matsuda, M.
iSicence 2018 Dec 21;10:98-113

11) ROSA26 reporter mouse lines and image analyses reveal the distinct region-specific cell behaviors in the visceral endoderm
Abe, T., Kutsuna, N., Kiyonari, H., Furuta, Y.,and Fujimori, T.
Development 2018 Nov 21;145(22)
PMID: 30327323

12) Dynamic organelle localization and cytoskeletal reorganization during preimplantation mouse embryo development revealed by live imaging of genetically encoded fluorescent fusion proteins
Kiyonari, H., Kaneko, M., Abe, T., Shioi, G., Aizawa, S., Furuta, Y., Fujimori, T.
genesis 2018 Feb; 57(2):e23277
PMID: 30597711

13) Tracing the origin of hair follicle setem cells
Morita,R., Sanzen, R., Sasaki, H., Hayashi, T., Umeda, M., Yoshimura, M., Yamamoto, T., Shibata, T., Abe, T., Kiyonari, H., Furuta, Y., Nikaido, I., Fujiwara, H.
Nature 2021 Jun;594(7864):547-552
PMID: 34108685