RIKEN Center for Life Science Technologies


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Labs & Technologies

Genetic Engineering Team

- Polishing GEMs (Genetically Engineered Mice) -
As Tools to Understand the Dynamics of Animal Development and Bio-Function


* Due to the reorganization starting as new centers in April 2018, this laboratory is now belong to the Center for Biosystems Dynamics Research. As for the latest information, please see the following URL below.
> The webpage of Laboratory for Genetic Engineering, Center for Biosystems Dynamics Research

Team Leader
Yasuhide Furuta  Ph.D.

2-2-3 Minatojima-minamimachi, Chuo-ku, Kobe, Hyogo 650-0047, Japan


>>> Lab Site

Research Area

Genetically engineered mice are one essential tool in modern biomedical research. The quality and efficacy of studies are greatly dependent on how efficiently mutant mice can be generated and propagated for various analyses. The major mission of the Genetic Engineering Team is to promote technical advancement of the generation of genetically engineered mouse models that are useful for biomedical research. In particular, our recent studies have focused on the development of new tools and technologies to understand biological dynamics by visualization of mouse development at the levels of tissue, cell, organelle, and developmental signaling. In addition, genome editing tools have bee utilized to aid in efficient generation of multi-gene mutant strains that can often been difficult to achieve in conventional genetic crosses. Using technical expertise and knowledge gained through these studies, in close coordination with Animal Resource Development Unit, our team works with research labs within the RIKEN Institutes and Centers, as well as others in Japan and throughout the Asia-Pacific region to develop genetically engineered mice useful for biomedical research. In these joint development projects, we receive sequence information of the subject genes from our collaborators, and perform all subsequent stages of mutant mouse production from construction of the targeting vectors to the generation of chimeras, making about one hundred new mutant mouse lines every year.

Main Publications List


A possible aid in targeted insertion of large DNA elements by CRISPR/Cas in mouse zygotes.

Nakao H, Harada T, Nakao K, Kiyonari H, Inoue K, Furuta Y, Aiba A.
Genesis., 54(2), 65-77 (2016).

CAMSAP3 orients the apical-to-basal polarity of microtubule arrays in epithelial cells

Toya M, Kobayashi S, Kawasaki M, Shioi G, Kaneko M, Ishiuchi T, Misaki K, Meng W, Takeichi M.
Proc Natl Acad Sci U S A, 113(2), 332-337 (2016).

Lats1 suppresses centrosome overduplication by modulating the stability of Cdc25B

Mukai S, Yabuta N, Yoshida K, Okamoto A, Miura D, Furuta Y, Abe T, Nojima H.
Sci Rep, 4(5), 16173 (2015).

Abrogation of protein phosphatase 6 promotes skin carcinogenesis induced by DMBA

Hayashi K, Momoi Y, Tanuma N, Kishimoto A, Ogoh H, Kato H, Suzuki M, Sakamoto Y, Inoue Y, Nomura M, Kiyonari H, Sakayori M, Fukamachi K, Kakugawa Y, Yamashita Y, Ito S, Sato I, Suzuki A, Nishio M, Suganuma M, Watanabe T, Shima H
Oncogene, 34(35), 4647-4655 (2015).

Quantitative Dynamics of Chromatin Remodeling during Germ Cell Specification from Mouse Embryonic Stem Cells

Kurimoto K, Yabuta Y, Hayashi K, Ohta H, Kiyonari H, Mitani T, Moritoki Y, Kohri K, Kimura H, Yamamoto T, Katou Y, Shirahige K, Saitou M.
Cell Stem Cell, 16(5), 517-532 (2015).

Whole-brain imaging with single-cell resolution using chemical cocktails and computational analysis.

Susaki EA, Tainaka K, Perrin D, Kishino F, Tawara T, Watanabe TM, Yokoyama C, Onoe H, Eguchi M, Yamaguchi S, Abe T, Kiyonari H, Shimizu Y, Miyawaki A, Yokota H, Ueda HR.
Cell, 157(3), 726-739 (2014).

Meiosis-specific cohesin mediates homolog recognition in mouse spermatocytes

Ishiguro K, Kim J, Shibuya H, Hernández-Hernández A, Suzuki A, Fukagawa T, Shioi G, Kiyonari H, Li XC, Schimenti J, Höög C, Watanabe Y.
Genes Dev, 28(6), 594-607 (2014).

Angiopoietin-1 guides directional angiogenesis through integrin αvβ5 signaling for recovery of ischemic retinopathy.

Lee J, Kim KE, Choi DK, Jang JY, Jung JJ, Kiyonari H, Shioi G, Chang W, Suda T, Mochizuki N, Nakaoka Y, Komuro I, Yoo OJ, Koh GY.
Sci Transl Med, 5, 203ra127 (2013).

Arid5a controls IL-6 mRNA stability, which contributes to elevation of IL-6 level in vivo

Masuda K, Ripley B, Nishimura R, Mino T, Takeuchi O, Shioi G, Kiyonari H, Kishimoto T.
Proc Natl Acad Sci U S A, 110(23), 9409-9414 (2013).

Reporter mouse lines for fluorescence imaging

Abe T, Fujimori T.
Dev Growth Differ., 55(4), 390-405 (2013).

Visualization of cell cycle in mouse embryos with Fucci2 reporter directed by Rosa26 promoter.

Abe T, Sakaue-Sawano A, Kiyonari H, Shioi G, Inoue K, Horiuchi T, Nakao K, Miyawaki A, Aizawa S, Fujimori T.
Development, 140(1), 237-246 (2013).

Member  *concurrent

CLST was reorganized into three centers according to the RIKEN 4th Medium-Term Plan from April 1, 2018. For the latest information of Genetic Engineering Team, please visit the following websites.

> The webpage of Laboratory for Genetic Engineering, Center for Biosystems Dynamics Research [http://www.bdr.riken.jp/en/research/labs/furuta-y/index.html]