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To let you know about our research, this area contains 4 types of information about CLST; “Article”, “Videos”, “Event” and “Study”.
At “Article”, you can read articles on interviews and lectures, and you can enjoy the videos about CLST at “Videos”. If you want to meet and talk directly with the researcher, “Visit” give you some information of such events. You can find more difficult contents to know about our research deeply at “Study”.
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Post-transcriptional Control Research Unit

Exploring the RNP complexes involved in the regulation of protein synthesis.

 

* Due to the reorganization starting as new centers in April 2018, the research activities of this laboratory are handed over to the Center for Biosystems Dynamics Research. As for the latest information, please see the following URL below.
> The webpage of Laboratory for Nonnatural Amino Acid Technology, Center for Biosystems Dynamics Research

Unit Leader
Motoaki Wakiyama  Ph.D.

1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan

1_4_wakiyama.png

Research Area

Control of cellular function and cell fate determination is dependent on the regulation of gene expression. The eukaryotic gene expression was thought to be mainly regulated at transcription level. However, based on the accumulation of the knowledge in microRNA function, it is now believed that post-transcriptional control of gene expression is much more diverse and intricate than previously thought, and is extensively involved in numerous diseases. Post-transcriptional gene regulation is mediated by a number of RNA-binding proteins (RBP), RBP-binding proteins and non-coding regulatory RNAs. These factors associate with the target mRNA molecule and form a huge mRNP complex. We aim to uncover the molecular mechanism of mRNA fate determination, by developing a novel method for the investigation of the architecture and function of mRNPs.

Main Publications List

1

MicroRNA-mediated deadenylation in a mammalian cell-free system.

Wakiyama M, Yokoyama S.
Methods Mol Biol, 1125, 341-351 (2014).
2

Posttranscriptional Control of Protein Synthesis in Drosophila S2 Cell-Free System

Wakiyama, M. and Yokoyama, S.
Methods Mol Biol, 1118, 257-266 (2014).
3

Tetrameric interaction of the ectoenzyme CD38 on the cell surface enables its catalytic and raft-association activities.

Hara-Yokoyama M, Kukimoto-Niino M, Terasawa K, Harumiya S, Podyma-Inoue KA, Hino N, Sakamoto K, Itoh S, Hashii N, Hiruta Y, Kawasaki N, Mishima-Tsumagari C, Kaitsu Y, Matsumoto T, Wakiyama M, Shirouzu M, Kasama T, Takayanagi H, Utsunomiya-Tate N, Takatsu K, Katada T, Hirabayashi Y, Yokoyama S, Yanagishita M.
Structure, 20(9), 1585-1592 (2012).
4

Structural basis for the altered drug sensitivities of non-small cell lung cancer-associated mutants of human epidermal growth factor receptor.

Yoshikawa S, Kukimoto-Niino M, Parker L, Handa N, Terada T, Fujimoto T, Terazawa Y, Wakiyama M, Sato M, Sano S, Kobayashi T, Tanaka T, Chen L, Liu ZJ, Wang BC, Shirouzu M, Kawa S, Semba K, Yamamoto T, Yokoyama S.
Oncogene, 32(1), 27-38 (2012).
5

Structures of the first and second double-stranded RNA-binding domains of human TAR RNA-binding protein.

Yamashita S, Nagata T, Kawazoe M, Takemoto C, Kigawa T, Güntert P, Kobayashi N, Terada T, Shirouzu M, Wakiyama M, Muto Y, Yokoyama S.
Protein Sci, 20(1), 118-130 (2011).
6

MicroRNA-mediated mRNA deadenylation and repression of protein synthesis in a mammalian cell-free system.

Wakiyama M, Yokoyama S.
Prog Mol Subcell Biol, 50, 85-97 (2010).
7

Mammalian GW182 contains multiple Argonaute-binding sites and functions in microRNA-mediated translational repression.

Takimoto K, Wakiyama M, Yokoyama S.
RNA, 15(6), 1078-1089 (2009).
8

Alteration of enzymatic properties of cell-surface antigen CD38 by agonistic anti-CD38 antibodies that prolong B cell survival and induce activation.

Hara-Yokoyama M, Kimura T, Kaku H, Wakiyama M, Kaitsu Y, Inoue M, Kusano S, Shirouzu M, Yokoyama S, Katada T, Hirabayashi Y, Takatsu K, Yanagishita M.
Int Immunopharmacol, 8(1), 59-70 (2008).
9

Let-7 microRNA-mediated mRNA deadenylation and translational repression in a mammalian cell-free system.

Wakiyama M, Takimoto K, Ohara O, Yokoyama S.
Genes Dev, 21(15), 1857-1862 (2007).
10

Cell-free translation system from Drosophila S2 cells that recapitulates RNAi.

Wakiyama M, Kaitsu Y, Yokoyama S.
Biochem Biophys Res Commun, 343(4), 1067-1071 (2006).

>>>ALL Publications

Member  *concurrent

  • Motoaki Wakiyama

    Unit Leader

  • Ryo Iwaoka

    Research Associate

  • Koichi Ogami

    Research Scientist

CLST was reorganized into three centers according to the RIKEN 4th Medium-Term Plan from April 1, 2018.


> The webpage of Laboratory for Nonnatural Amino Acid Technology, Center for Biosystems Dynamics Research [http://www.bdr.riken.jp/en/research/labs/sakamoto-k/index.html]


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