RIKEN Center for Life Science Technologies

About CLST

Idea
greeting

Jobs at CLST

Access

Contact us

Links

Labs

Labs & Program

Center Director's Strategic Program RIKEN CLST-JEOL Collaboration Center

Publications

FANTOM

Technologies & Facilities

Technologies of CLST

Service of CLST

Radiosynthesis Database of PET Probes

Topics

  • Research

    Latest publications from CLST.
     

  • News

    Awards, agreements, and other news.

  • Events

    Upcoming and past events including seminars, symposiums, open days.

For Public

To let you know about our research, this area contains 4 types of information about CLST; “Article”, “Videos”, “Event” and “Study”.
At “Article”, you can read articles on interviews and lectures, and you can enjoy the videos about CLST at “Videos”. If you want to meet and talk directly with the researcher, “Visit” give you some information of such events. You can find more difficult contents to know about our research deeply at “Study”.
  • Article
  • Event
  • Videos
  • Study

Labs & Technologies

Translation Factors Structural Biology Unit

Reveals the mechanisms of translation

 

* Due to the reorganization starting as new centers in April 2018, this laboratory is now belong to the Center for Biosystems Dynamics Research. As for the latest information, please see the following URL below.
> The webpage of Laboratory for Translation Structural Biology, Center for Biosystems Dynamics Research

Unit Leader
Takuhiro Ito  Ph.D.

Room N217, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama City, Kanagawa 230-0045, Japan
Tel: +81-45-503-9204

1_5_ito.png

Research Area

We plan to conduct studies on three dimensional structures of the translation factors and/or the ribosome, mainly by X-ray crystallography, focusing on the structural analysis of the large complexes that function in the eukaryotic translational system, in order to reveal the complicated mechanisms of eukaryotic translation.

Main Publications List

1

Crystal structure of eukaryotic translation initiation factor 2B

Kashiwagi K, Takahashi M, Nishimoto M, Hiyama TB, Higo T, Umehara T, Sakamoto K, Ito T, Yokoyama S
Nature, 351(7592), 122-125 (2016).
2

Expression, purification, and crystallization of Schizosaccharomyces pombe eIF2B

Kashiwagi K, Shigeta T, Imataka H, Ito T, Yokoyama S
J Struct Funct Genomics, 17(1), 33-38 (2016).
3

Structural basis for methyl-donor-dependent and sequence-specific binding to tRNA substrates by knotted methyltransferase TrmD.

Ito T, Masuda I, Yoshida K, Goto-Ito S, Sekine S, Suh SW, Hou YM, Yokoyama S.
Proc Natl Acad Sci USA., 112(31), E4197-205 (2015).
4

RBFOX and SUP-12 sandwich a G base to cooperatively regulate tissue-specific splicing

Kuwasako K, Takahashi M, Unzai S, Tsuda K, Yoshikawa S, He F, Kobayashi N, Gntert P, Shirouzu M, Ito T, Tanaka A, Yokoyama S, Hagiwara M, Kuroyanagi H, Muto Y.
Nat Struct Mol Biol, 21(9), 778-786 (2014).
5

Crystal structure of the eukaryotic translation initiation factor 2A from Schizosaccharomyces pombe

Kashiwagi K, Ito T, Yokoyama S.
J Struct Funct Genomics, 15(3), 125-130 (2013).
6

Differentiating analogous tRNA methyltransferases by fragments of the methyl donor.

Lahoud G, Goto-Ito S, Yoshida K, Ito T, Yokoyama S, Hou YM.
RNA, 17(7), 1236-1246 (2011).
7

Two enzymes bound to one transfer RNA assume alternative conformations for consecutive reactions.

Ito T, Yokoyama S.
Nature, 467(7315), 612-616 (2010).
8

Structure of nondiscriminating glutamyl-tRNA synthetase from Thermotoga maritima.

Ito T, Kiyasu N, Matsunaga R, Takahashi S, Yokoyama S.
Acta Crystallogr Sect D-Biol Crystallogr, 66(Pt7), 813-820 (2010).
9

Tertiary structure checkpoint at anticodon loop modification in tRNA functional maturation.

Goto-Ito S, Ito T, Kuratani M, Bessho Y, Yokoyama S.
Nat Struct Mol Biol, 16(10), 1109-1115 (2009).
10

Crystal structure of the alpha subunit of human translation initiation factor 2B.

Hiyama TB, Ito T, Imataka H, Yokoyama S.
J Mol Biol, 392(4), 937-951 (2009).

Member  *concurrent

  • Takuhiro Ito

    Unit Leader

    >>>Curriculum vitae
  • Kazuhiro Kashiwagi

    Research Scientist

  • Madoka Nishimoto

    Technical Scientist

  • Mari Takahashi

    Research Associate

Links

CLST was reorganized into three centers according to the RIKEN 4th Medium-Term Plan from April 1, 2018. For the latest information of Translation Factors Structural Biology Unit, please visit the following websites.


> The webpage of Laboratory for Translation Structural Biology, Center for Biosystems Dynamics Research [http://www.bdr.riken.jp/en/research/labs/ito-t/index.html]


> If you continue to browse this site, click here.